Ubiquitin Proteomics:Article Title: RUNX2 and USP16 stabilize MFRN2 to maintain pulmonary epithelial barrier integrity in sepsis-induced acute lung injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ZO-1 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 Occludin Proteintech Cat# 27260-1-AP; RRID: AB_2880820 Claudin3 Proteintech Cat# 16456-1-AP; RRID: AB_10733226 Claudin18 Proteintech Cat# 21126-1-AP; AB_10733638 4-HNE Abcam Cat# ab46545; RRID: AB_722490 ACSL4 Cell Signaling technology (CST) Cat# 38493; RRID: AB_3719913 GPX4 Abclonal Cat# A27995; RRID: AB_3740908 HO-1 Abclonal Cat# A28140PM; RRID: AB_3740909 SLC7A11 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 MFRN2 Abmart Cat# PC7157; RRID: AB_3740907 RUNX2 Proteintech Cat# 20700-1-AP; RRID: AB_2722783 USP16 Abcam Cat# ab236628; RRID: AB_3740912 Ubiquitin Abcam Cat# ab134953; RRID: AB_2801561 GAPDH Abcam Cat# ab181602; RRID: AB_2630358 Horseradish peroxidase (HRP)-coupled goat anti-mouse/rabbit IgG Abcam Cat# ab6789, ab6721; RRID: AB_955439, AB_955447 Alexa Fluor 488- and 568-conjugated secondary antibodies Abcam Cat# ab150125, ab150127; RRID: AB_3740910, AB_3740911 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Pseudomonas Aeruginosa ATCC Cat# ACS-27853 Chemicals, peptides, and recombinant proteins RUNX2 recombinant proteins Abcam Cat # ab112259 Heparin Sigma Cat #4784 DAPI Sigma Cat #S7113 Hoechst 33258 Beyotime Cat# C1018 MG132 MCE Cat# HY-13259 Ammonium chloride MCE Cat #HY-Y1269 Cycloheximide MCE Cat #HY-12320 3-Methyladenine MCE Cat #HY-19312 Tamoxifen Sigma Cat #T5648 FBS Gibco Cat #A5256701 Ham’s F-12K Gibco Cat #21127022 RPMI-1640 Gibco Cat #11875093 PBS Gibco Cat #10010023 Lipopolysaccharide Sigma Cat #L2630 Deposited data Raw and analyzed data of bulk RNA-seq This paper CRA038604 IP-MS proteomics data This paper PXD074209 The original immunoblots for Figures 1, 2, 3, 4, 5, 6, S1, S2, and S4–S8 This paper data.mendeley: https://doi.org/ 10.17632/4byfwttyzd.1 Experimental models: Cell lines HEK293T cells ATCC Cat # CRL-11268 A549 cells ATCC Cat # CRM-CCL-185 (Continued on next page) 14 Cell Reports 45, 117184, April 28, 2026 .. All animal care and experimental procedures were conducted in accordance with the guidelines of the National Institutes of Health (NIH) for the care and use of laboratory animals and approved by the Animal Welfare and Ethics Committee of Renmin Hospital of Wuhan University (NO.20240502A), Wuhan, China.
Virus:Article Title: RUNX2 and USP16 stabilize MFRN2 to maintain pulmonary epithelial barrier integrity in sepsis-induced acute lung injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ZO-1 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 Occludin Proteintech Cat# 27260-1-AP; RRID: AB_2880820 Claudin3 Proteintech Cat# 16456-1-AP; RRID: AB_10733226 Claudin18 Proteintech Cat# 21126-1-AP; AB_10733638 4-HNE Abcam Cat# ab46545; RRID: AB_722490 ACSL4 Cell Signaling technology (CST) Cat# 38493; RRID: AB_3719913 GPX4 Abclonal Cat# A27995; RRID: AB_3740908 HO-1 Abclonal Cat# A28140PM; RRID: AB_3740909 SLC7A11 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 MFRN2 Abmart Cat# PC7157; RRID: AB_3740907 RUNX2 Proteintech Cat# 20700-1-AP; RRID: AB_2722783 USP16 Abcam Cat# ab236628; RRID: AB_3740912 Ubiquitin Abcam Cat# ab134953; RRID: AB_2801561 GAPDH Abcam Cat# ab181602; RRID: AB_2630358 Horseradish peroxidase (HRP)-coupled goat anti-mouse/rabbit IgG Abcam Cat# ab6789, ab6721; RRID: AB_955439, AB_955447 Alexa Fluor 488- and 568-conjugated secondary antibodies Abcam Cat# ab150125, ab150127; RRID: AB_3740910, AB_3740911 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Pseudomonas Aeruginosa ATCC Cat# ACS-27853 Chemicals, peptides, and recombinant proteins RUNX2 recombinant proteins Abcam Cat # ab112259 Heparin Sigma Cat #4784 DAPI Sigma Cat #S7113 Hoechst 33258 Beyotime Cat# C1018 MG132 MCE Cat# HY-13259 Ammonium chloride MCE Cat #HY-Y1269 Cycloheximide MCE Cat #HY-12320 3-Methyladenine MCE Cat #HY-19312 Tamoxifen Sigma Cat #T5648 FBS Gibco Cat #A5256701 Ham’s F-12K Gibco Cat #21127022 RPMI-1640 Gibco Cat #11875093 PBS Gibco Cat #10010023 Lipopolysaccharide Sigma Cat #L2630 Deposited data Raw and analyzed data of bulk RNA-seq This paper CRA038604 IP-MS proteomics data This paper PXD074209 The original immunoblots for Figures 1, 2, 3, 4, 5, 6, S1, S2, and S4–S8 This paper data.mendeley: https://doi.org/ 10.17632/4byfwttyzd.1 Experimental models: Cell lines HEK293T cells ATCC Cat # CRL-11268 A549 cells ATCC Cat # CRM-CCL-185 (Continued on next page) 14 Cell Reports 45, 117184, April 28, 2026 .. All animal care and experimental procedures were conducted in accordance with the guidelines of the National Institutes of Health (NIH) for the care and use of laboratory animals and approved by the Animal Welfare and Ethics Committee of Renmin Hospital of Wuhan University (NO.20240502A), Wuhan, China.
Article Title: H3K27-specific demethylase orchestrates the phenotypic and functional plasticity of intestinal ILC3s.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: organisms/strains Mouse:Kdm6a flox/flox (Utx) Jackson Laboratories Strain #:021926 RRID:IMSR_JAX:021926 Mouse:Kdm6b flox/flox (Jmjd3) Jackson Laboratories Strain #:029615 RRID:IMSR_JAX:029615 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Deposited data RNAseq data This paper CRA033355 CUT&Tag This paper CRA033268 (Continued on next page) Cell Reports 45, 116862, January 27, 2026 17
Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER IRF-4 Rabbit mAb (D9P5H) Cell Signaling Cat# 15106T; RRID: AB_2798709 Anti-BATF (EPR21911) Abcam Cat# ab221146; RRID: AB_3665801 Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; RRID:AB_1031062 Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; RRID: AB_3697514 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: Cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: Organisms/strains Mouse:Irf4 flox/flox Jackson Laboratories Strain #:009380 RRID:IMSR_JAX:009380 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Mouse:OT-II Jackson Laboratories Strain #:004194 RRID:IMSR_JAX:004194 (Continued on next page) e2 iScience 28, 112800, July 18, 2025
Recombinant:Article Title: RUNX2 and USP16 stabilize MFRN2 to maintain pulmonary epithelial barrier integrity in sepsis-induced acute lung injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ZO-1 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 Occludin Proteintech Cat# 27260-1-AP; RRID: AB_2880820 Claudin3 Proteintech Cat# 16456-1-AP; RRID: AB_10733226 Claudin18 Proteintech Cat# 21126-1-AP; AB_10733638 4-HNE Abcam Cat# ab46545; RRID: AB_722490 ACSL4 Cell Signaling technology (CST) Cat# 38493; RRID: AB_3719913 GPX4 Abclonal Cat# A27995; RRID: AB_3740908 HO-1 Abclonal Cat# A28140PM; RRID: AB_3740909 SLC7A11 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 MFRN2 Abmart Cat# PC7157; RRID: AB_3740907 RUNX2 Proteintech Cat# 20700-1-AP; RRID: AB_2722783 USP16 Abcam Cat# ab236628; RRID: AB_3740912 Ubiquitin Abcam Cat# ab134953; RRID: AB_2801561 GAPDH Abcam Cat# ab181602; RRID: AB_2630358 Horseradish peroxidase (HRP)-coupled goat anti-mouse/rabbit IgG Abcam Cat# ab6789, ab6721; RRID: AB_955439, AB_955447 Alexa Fluor 488- and 568-conjugated secondary antibodies Abcam Cat# ab150125, ab150127; RRID: AB_3740910, AB_3740911 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Pseudomonas Aeruginosa ATCC Cat# ACS-27853 Chemicals, peptides, and recombinant proteins RUNX2 recombinant proteins Abcam Cat # ab112259 Heparin Sigma Cat #4784 DAPI Sigma Cat #S7113 Hoechst 33258 Beyotime Cat# C1018 MG132 MCE Cat# HY-13259 Ammonium chloride MCE Cat #HY-Y1269 Cycloheximide MCE Cat #HY-12320 3-Methyladenine MCE Cat #HY-19312 Tamoxifen Sigma Cat #T5648 FBS Gibco Cat #A5256701 Ham’s F-12K Gibco Cat #21127022 RPMI-1640 Gibco Cat #11875093 PBS Gibco Cat #10010023 Lipopolysaccharide Sigma Cat #L2630 Deposited data Raw and analyzed data of bulk RNA-seq This paper CRA038604 IP-MS proteomics data This paper PXD074209 The original immunoblots for Figures 1, 2, 3, 4, 5, 6, S1, S2, and S4–S8 This paper data.mendeley: https://doi.org/ 10.17632/4byfwttyzd.1 Experimental models: Cell lines HEK293T cells ATCC Cat # CRL-11268 A549 cells ATCC Cat # CRM-CCL-185 (Continued on next page) 14 Cell Reports 45, 117184, April 28, 2026 .. All animal care and experimental procedures were conducted in accordance with the guidelines of the National Institutes of Health (NIH) for the care and use of laboratory animals and approved by the Animal Welfare and Ethics Committee of Renmin Hospital of Wuhan University (NO.20240502A), Wuhan, China.
RNA Sequencing:Article Title: RUNX2 and USP16 stabilize MFRN2 to maintain pulmonary epithelial barrier integrity in sepsis-induced acute lung injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ZO-1 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 Occludin Proteintech Cat# 27260-1-AP; RRID: AB_2880820 Claudin3 Proteintech Cat# 16456-1-AP; RRID: AB_10733226 Claudin18 Proteintech Cat# 21126-1-AP; AB_10733638 4-HNE Abcam Cat# ab46545; RRID: AB_722490 ACSL4 Cell Signaling technology (CST) Cat# 38493; RRID: AB_3719913 GPX4 Abclonal Cat# A27995; RRID: AB_3740908 HO-1 Abclonal Cat# A28140PM; RRID: AB_3740909 SLC7A11 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 MFRN2 Abmart Cat# PC7157; RRID: AB_3740907 RUNX2 Proteintech Cat# 20700-1-AP; RRID: AB_2722783 USP16 Abcam Cat# ab236628; RRID: AB_3740912 Ubiquitin Abcam Cat# ab134953; RRID: AB_2801561 GAPDH Abcam Cat# ab181602; RRID: AB_2630358 Horseradish peroxidase (HRP)-coupled goat anti-mouse/rabbit IgG Abcam Cat# ab6789, ab6721; RRID: AB_955439, AB_955447 Alexa Fluor 488- and 568-conjugated secondary antibodies Abcam Cat# ab150125, ab150127; RRID: AB_3740910, AB_3740911 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Pseudomonas Aeruginosa ATCC Cat# ACS-27853 Chemicals, peptides, and recombinant proteins RUNX2 recombinant proteins Abcam Cat # ab112259 Heparin Sigma Cat #4784 DAPI Sigma Cat #S7113 Hoechst 33258 Beyotime Cat# C1018 MG132 MCE Cat# HY-13259 Ammonium chloride MCE Cat #HY-Y1269 Cycloheximide MCE Cat #HY-12320 3-Methyladenine MCE Cat #HY-19312 Tamoxifen Sigma Cat #T5648 FBS Gibco Cat #A5256701 Ham’s F-12K Gibco Cat #21127022 RPMI-1640 Gibco Cat #11875093 PBS Gibco Cat #10010023 Lipopolysaccharide Sigma Cat #L2630 Deposited data Raw and analyzed data of bulk RNA-seq This paper CRA038604 IP-MS proteomics data This paper PXD074209 The original immunoblots for Figures 1, 2, 3, 4, 5, 6, S1, S2, and S4–S8 This paper data.mendeley: https://doi.org/ 10.17632/4byfwttyzd.1 Experimental models: Cell lines HEK293T cells ATCC Cat # CRL-11268 A549 cells ATCC Cat # CRM-CCL-185 (Continued on next page) 14 Cell Reports 45, 117184, April 28, 2026 .. All animal care and experimental procedures were conducted in accordance with the guidelines of the National Institutes of Health (NIH) for the care and use of laboratory animals and approved by the Animal Welfare and Ethics Committee of Renmin Hospital of Wuhan University (NO.20240502A), Wuhan, China.
Protein-Protein interactions:Article Title: RUNX2 and USP16 stabilize MFRN2 to maintain pulmonary epithelial barrier integrity in sepsis-induced acute lung injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ZO-1 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 Occludin Proteintech Cat# 27260-1-AP; RRID: AB_2880820 Claudin3 Proteintech Cat# 16456-1-AP; RRID: AB_10733226 Claudin18 Proteintech Cat# 21126-1-AP; AB_10733638 4-HNE Abcam Cat# ab46545; RRID: AB_722490 ACSL4 Cell Signaling technology (CST) Cat# 38493; RRID: AB_3719913 GPX4 Abclonal Cat# A27995; RRID: AB_3740908 HO-1 Abclonal Cat# A28140PM; RRID: AB_3740909 SLC7A11 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 MFRN2 Abmart Cat# PC7157; RRID: AB_3740907 RUNX2 Proteintech Cat# 20700-1-AP; RRID: AB_2722783 USP16 Abcam Cat# ab236628; RRID: AB_3740912 Ubiquitin Abcam Cat# ab134953; RRID: AB_2801561 GAPDH Abcam Cat# ab181602; RRID: AB_2630358 Horseradish peroxidase (HRP)-coupled goat anti-mouse/rabbit IgG Abcam Cat# ab6789, ab6721; RRID: AB_955439, AB_955447 Alexa Fluor 488- and 568-conjugated secondary antibodies Abcam Cat# ab150125, ab150127; RRID: AB_3740910, AB_3740911 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Pseudomonas Aeruginosa ATCC Cat# ACS-27853 Chemicals, peptides, and recombinant proteins RUNX2 recombinant proteins Abcam Cat # ab112259 Heparin Sigma Cat #4784 DAPI Sigma Cat #S7113 Hoechst 33258 Beyotime Cat# C1018 MG132 MCE Cat# HY-13259 Ammonium chloride MCE Cat #HY-Y1269 Cycloheximide MCE Cat #HY-12320 3-Methyladenine MCE Cat #HY-19312 Tamoxifen Sigma Cat #T5648 FBS Gibco Cat #A5256701 Ham’s F-12K Gibco Cat #21127022 RPMI-1640 Gibco Cat #11875093 PBS Gibco Cat #10010023 Lipopolysaccharide Sigma Cat #L2630 Deposited data Raw and analyzed data of bulk RNA-seq This paper CRA038604 IP-MS proteomics data This paper PXD074209 The original immunoblots for Figures 1, 2, 3, 4, 5, 6, S1, S2, and S4–S8 This paper data.mendeley: https://doi.org/ 10.17632/4byfwttyzd.1 Experimental models: Cell lines HEK293T cells ATCC Cat # CRL-11268 A549 cells ATCC Cat # CRM-CCL-185 (Continued on next page) 14 Cell Reports 45, 117184, April 28, 2026 .. All animal care and experimental procedures were conducted in accordance with the guidelines of the National Institutes of Health (NIH) for the care and use of laboratory animals and approved by the Animal Welfare and Ethics Committee of Renmin Hospital of Wuhan University (NO.20240502A), Wuhan, China.
Western Blot:Article Title: RUNX2 and USP16 stabilize MFRN2 to maintain pulmonary epithelial barrier integrity in sepsis-induced acute lung injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ZO-1 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 Occludin Proteintech Cat# 27260-1-AP; RRID: AB_2880820 Claudin3 Proteintech Cat# 16456-1-AP; RRID: AB_10733226 Claudin18 Proteintech Cat# 21126-1-AP; AB_10733638 4-HNE Abcam Cat# ab46545; RRID: AB_722490 ACSL4 Cell Signaling technology (CST) Cat# 38493; RRID: AB_3719913 GPX4 Abclonal Cat# A27995; RRID: AB_3740908 HO-1 Abclonal Cat# A28140PM; RRID: AB_3740909 SLC7A11 Proteintech Cat# 21773-1-AP; RRID: AB_10733242 MFRN2 Abmart Cat# PC7157; RRID: AB_3740907 RUNX2 Proteintech Cat# 20700-1-AP; RRID: AB_2722783 USP16 Abcam Cat# ab236628; RRID: AB_3740912 Ubiquitin Abcam Cat# ab134953; RRID: AB_2801561 GAPDH Abcam Cat# ab181602; RRID: AB_2630358 Horseradish peroxidase (HRP)-coupled goat anti-mouse/rabbit IgG Abcam Cat# ab6789, ab6721; RRID: AB_955439, AB_955447 Alexa Fluor 488- and 568-conjugated secondary antibodies Abcam Cat# ab150125, ab150127; RRID: AB_3740910, AB_3740911 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Pseudomonas Aeruginosa ATCC Cat# ACS-27853 Chemicals, peptides, and recombinant proteins RUNX2 recombinant proteins Abcam Cat # ab112259 Heparin Sigma Cat #4784 DAPI Sigma Cat #S7113 Hoechst 33258 Beyotime Cat# C1018 MG132 MCE Cat# HY-13259 Ammonium chloride MCE Cat #HY-Y1269 Cycloheximide MCE Cat #HY-12320 3-Methyladenine MCE Cat #HY-19312 Tamoxifen Sigma Cat #T5648 FBS Gibco Cat #A5256701 Ham’s F-12K Gibco Cat #21127022 RPMI-1640 Gibco Cat #11875093 PBS Gibco Cat #10010023 Lipopolysaccharide Sigma Cat #L2630 Deposited data Raw and analyzed data of bulk RNA-seq This paper CRA038604 IP-MS proteomics data This paper PXD074209 The original immunoblots for Figures 1, 2, 3, 4, 5, 6, S1, S2, and S4–S8 This paper data.mendeley: https://doi.org/ 10.17632/4byfwttyzd.1 Experimental models: Cell lines HEK293T cells ATCC Cat # CRL-11268 A549 cells ATCC Cat # CRM-CCL-185 (Continued on next page) 14 Cell Reports 45, 117184, April 28, 2026 .. All animal care and experimental procedures were conducted in accordance with the guidelines of the National Institutes of Health (NIH) for the care and use of laboratory animals and approved by the Animal Welfare and Ethics Committee of Renmin Hospital of Wuhan University (NO.20240502A), Wuhan, China.
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